RNA Preparation and Purification
ATCC Synthetic Zika; virus RNA
Quantitative synthetic RNA for Zika virus can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. This preparation includes fragments from the Membrane glycoprotein precursor M, Envelope, NS1, NS2B, NS3, NS4B, and NS5 regions.
Active Motif CUT&Tag-IT R-loop Kit 16 rxns
It is easy to profile DNA-RNA hybrid R-loops genome-wide with Active Motif’s CUT&Tag-IT™ R-loop Assay Kit. The kit utilizes a CUT&Tag-based approach to profile R-loops genome-wide using a specifically optimized protocol for cell samples and our DNA-RNA hybrid (Clone S9.6) antibody. This is not simply the same as our CUT&Tag-IT Assay Kit with a DNA-RNA Hybrid mAb (Clone S9.6). The CUT&Tag-IT R-loop Assay Kit includes optimized DNA Binding Buffer, modified DNA Purification Columns, Q5 Polymerase Master Mix and a second mixing step during secondary antibody incubation all performed in an efficient strip-tube format protocol specifically designed for R-loop targets.
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 24 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
Bioworld RNase-Off Refill, 1 L
RNase Off Refill, 500MLUnique formulation destroys RNase & DNase on contact Completely washed away when rinsed Spray container prevents waste of reagents and uniformly covers area Does not contain any toxic agents or leave a residue
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) – 96 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.
System Biosciences LLC SERAMIR EXOSOME RNA PURIF KIT
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns) 20 preps
Macherey-Nagel NUCLEOSPIN 96 RNA BLD 96WL KIT
NucleoSpin 96 RNA Blood, 96-well kit for RNA from blood
Zymo Research Corporation ZR RNA Buffer, 50 ml
ZR RNA Buffer is a one-step lysis and binding buffer for the purification of RNA in the Quick-RNA Midiprep Kit.
![Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502037264.JPG-250.jpg)
Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]
The extraction method inactivates viruses and other infectious agents. Total RNA (including small/microRNAs) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, DNA/RNA Shield™ samples, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRIzol/TRI Reagent, wash and elute the RNA. No chloroform, phase separation or precipitation steps are necessary. High-quality total RNA is ready for Next-Gen sequencing, RT-qPCR, transcription profiling, hybridization, etc
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Hippocampus, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.