RNA Preparation and Purification
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 96 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
Agilent Technologies ABSOLUTELY RNA NANOPREP KITAB
Absolutely RNA Nanoprep Kit 50 preps The Absolutely RNA nanoprep kit offers easy purification of DNA-free total RNA from the smallest samples eluted in a volume of 10microliter to provide RNA at a concentration useful for many downstream ap
Active Motif CUT&Tag-IT R-loop Kit 16 rxns
It is easy to profile DNA-RNA hybrid R-loops genome-wide with Active Motif’s CUT&Tag-IT™ R-loop Assay Kit. The kit utilizes a CUT&Tag-based approach to profile R-loops genome-wide using a specifically optimized protocol for cell samples and our DNA-RNA hybrid (Clone S9.6) antibody. This is not simply the same as our CUT&Tag-IT Assay Kit with a DNA-RNA Hybrid mAb (Clone S9.6). The CUT&Tag-IT R-loop Assay Kit includes optimized DNA Binding Buffer, modified DNA Purification Columns, Q5 Polymerase Master Mix and a second mixing step during secondary antibody incubation all performed in an efficient strip-tube format protocol specifically designed for R-loop targets.
Research Products International Corp Quick-RNA MiniPrep Kit, w/Zymo-Spin IIICG Columns, Capped, & Spin-Away Filters, 200 Preps
The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) with Sample Purification Beads – 24 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA. For convenience, this kit includes RNA Clean beads.
Research Products International Corp Quick-RNA MicroPrep Kit w/Zymo-Spin IC Columns, Capped, 200 Preps
The Quick-RNA Microprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: DNase I included.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
New England Biolabs, Inc. NEBNext RNA Depletion Core Reagent Set - 6 rxns
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool. The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. The kit is also available with RNAClean beads.
Beckman Coulter Wash WBD reagent (C39489)
Beckman Coulter Wash WBD (C39489) is a liquid wash reagent used with Beckman Coulter diagnostic analyzers in automated wash cycles to remove residual reagents and help maintain assay performance.
- Provides instrument wash for automated analyzers.
- Helps remove residual reagent and reduce carryover.
- Intended for laboratory diagnostic use.
- Supplied as a liquid reagent for use in instrument wash systems.